Evaluation of hematological and biochemical parameters of liver function following consumption of Vitex doniana fruit syrup

Fred Coolborn Akharaiyi; Chioma Bertha Ehis-Eriokha; Peace Omoikhudu Oleghe; Lucky Efe Isunu

doi:10.51745/najfnr.8.18.229-240

Evaluation of hematological and biochemical parameters of liver function following consumption of Vitex doniana fruit syrup

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Food Microbiology, Safety and Toxicology Human and Clinical Nutrition

DOI https://doi.org/10.51745/najfnr.8.18.229-240

Issue Vol. 8 No. 18 (2024): July - December, 2024

Submitted 2024-07-09

Accepted 2024-12-07

Published 2024-12-08

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Background: Vitex doniana is a tropical plant whose leaves and fruits are traditionally utilized for medicinal and nutritional purposes.

Aims: This study aimed to evaluate the hematological and biochemical effects of Vitex doniana fruit syrup on liver function using an animal model.

Subjects and Methods: Male and female mice with weighing between 24 – 32 g were utilized in this study. Group 1 served as the negative control and was provided with feed and water. Groups 2 – 6 were orally administered single doses of the syrup at concentrations of 25, 30, 35, 40, and 45 mL for 14 days. Hematological parameters were analyzed using an automated hematologic analyzer. In vivo antioxidant and biochemical assays were conducted using standard chemical methods, while histopathological assessments were performed using hematoxylin and eosin staining.

Results: The mice showed a weight gain ranging from 8.12 to 9.81 g over the study period. An increase was observed in red blood cell count (7.32 – 7.45 m/cu.mm), white blood cells (4.30 – 4.35 t/cu.mm), lymphocytes (55.21 – 54.72%), neutrophils (21.64 – 12.70%), and packed cell volume (PCV) (41.04 – 41.16%). Antioxidant activity showed catalase values between 50.20–58.21 µM/g, glutathione (23.41 – 28.34 µM/g), and lipid peroxidation levels of 93.54 – 106.21 µM/g). Biochemical analysis revealed a reduction in alkaline phosphate (91.17 – 85.24 IU/L), aspartate aminotransferase (58.67 – 31.56 IU/L), and alanine aminotransferase (58.16 to 43.36 IU/L). Decreases were also observed in total bilirubin, creatinine, cholesterol, and urea levels. However, an increase in total albumin (3.16 – 3.38 mg/dL), total protein (6.42 – 6.74 mg/dL), and uric acid (7.06 – 5.10 mg/dL) was recorded with increasing syrup concentrations.

Conclusion: The study concludes that Vitex doniana fruit syrup poses no adverse health implications. On the contrary, it exhibits potential for blood maintenance, antioxidant activity, and supports enzymatic functions beneficial for liver health.

Keywords: Antioxidants, biomarkers, hematology, liver function, syrup, V. doniana

Fred Coolborn Akharaiyi

Edo State University Uzairue Faculty of Science, Department of Microbiology Km 7 Auchi-Abuja Road, Edo State
Chioma Bertha Ehis-Eriokha

Edo State University Uzairue Faculty of Science, Department of Microbiology Km 7 Auchi-Abuja Road, Edo State
Peace Omoikhudu Oleghe

Department of Biological Science Laboratory Technology, School of Applied Sciences and Technology, Auchi Polytechnic, Auchi, P.M.B, 13, Auchi, Edo State,
Lucky Efe Isunu

Department of Microbiology, Federal University of Technology, P. M. B. 704, Akure, Ondo State

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Evaluation of hematological and biochemical parameters of liver function following consumption of Vitex doniana fruit syrup. (2024). The North African Journal of Food and Nutrition Research, 8(18), 229-240. https://doi.org/10.51745/najfnr.8.18.229-240

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Abel, L., Levy, B. B., Brodie, B. B., & Kendall, F. E. (1952). A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. Journal of Biological Chemistry, 195(1), 357–66.

Adjei, S., Amponsah, I. K., Bekoe, S. O., Harley, B. K., Mensah, K. B., Mensah, A. Y., Baah, M. K., & Fosu-Mensah, G. (2021). Fruits of Vitex doniana sweet: toxicity profile, anti-inflammatory and antioxidant activities, and quantification of one of its bioactive constituents oleanolic acid. Heliyon, 7(9), e07910. https://doi.org/10.1016/j.heliyon.2021.e07910

Aiwonegbe, A. E, Iyasele, J. U., & Izevbuwa, N. O (2018). Proximate composition, phytochemical and antimicrobial screening of the methanol and acetone extracts of Vitex doniana fruit pulp. IFE Journal of Science, 20(2), 317–323.

Akharaiyi, F. C., & Maliki, M (2023). Inhibition of bacterial growth, mineral, proximate and vitamin contents of a syrup prepared from Vitex doniana fruits. Infection Epidemiology and Microbiology, 9(2), 137-147. https://doi.org/10.52547/iem.9.2.137

Akharaiyi, F. C., & Okafor, A. C. (2021). Effect of Spathodea campanulata Ethanol Leaf Extract on Hematology and Liver Function of Salmonella infected and Paracetamol-induced Swiss Albino Mice. FABAD Journal of Pharmaceutical Science, 46(3), 251–260.

Akharaiyi, Fred Coolborn, Imarhiagbe, O., Isunu, L. E., & Ajibola, A. T. (2022). Ethanol leaf extract of Hoslundia opposita in in vivo antioxidant and hepatoprotective activity using an animal model. BioMedicine, 12(3), 48–55. https://doi.org/10.37796/2211-8039.1321

Amuzat, A. O., Ndatsu, Y., Adisa, M. J., Sulaiman, R. S., Mohammed, H., Yusuf, A. A., & Ndayako, H. M. (2020). Anti-diarrhoeal Effects of Aqueous Extract of Vitex doniana Stem Bark in. Castor Oil-induced Wistar Rats. Tanzania Journal of Science, 46(3), 723–732. https://dx.doi.org/10.4314/tjs.v46i3.13

Ayoka, T. O., Nwanchukwu, N., Aloysius, C. E., Chidi, U. I., & Anadi C. O. (2023). Hepatocurative and histopathological evaluations in albino Rats exposed to Vitex doniana alkaloids. Letter in Applied Nanobioscience, 12(2), 56 https://doi.org/10.33263/lianbs122.056

Bain, B. J., Bates, I., Laffan, M. A., & Mitchell Lewis, S. (2011). Dacie and Lewis practical haematology E-book: Expert consult: Online and print (11th ed.). Churchill Livingstone.

Barry, P. R., Sanou, A., Konaté, K., Aworet-Samseny, R. R., Sytar, O., & Dicko, M. H. (2022). Toxicological profile, phytochemical analysis and anti-inflammatory properties of leaves of Vitex doniana Sweet. (Verbenaceae). Heliyon, 8(8), e10080. https://doi.org/10.1016/j.heliyon.2022.e10080

Benmohamed, M., Guenane, H., Messaoudi, M., Zahnit, W., Egbuna, C., Sharifi-Rad, M., Chouh, A., Seghir, B. B., Rebiai, A., Boubekeur, S., Azli, T., Harrat, M., Sawicka, B., Atanassova, M., & Yousfi, M. (2023). Mineral profile, antioxidant, anti-inflammatory, antibacterial, anti-urease and anti-α-amylase activities of the unripe fruit extracts of Pistacia atlantica. Molecules (Basel, Switzerland), 28(1), 349. https://doi.org/10.3390/molecules28010349

Bergmeyer, H. U., Horder, M., & Rej, R. (1985a). International Federation of Clinical Chemistry (IFCC) Scientific Committee, Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 2. IFCC method for aspartate aminotransferase (L-aspartate: 2- oxoglutarate aminotransferase, EC 2.6 1:1.). Journal of Clinical Chemistry and Clinical Biochemistry, 24, 497–510.

Bergmeyer, H. U., Horder, M., & Rej, R. I (1985b) International Federation of Clinical Chemistry (IFCC) Scientific Committee, Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase (L-alanine: 2-oxoglutarate aminotransferase, EC 2.6 1:2.). Journal of Clinical Chemistry and Clinical Biochemistry, 24, 481-495.

Bolanle, J. D., Adetoro, K. O., Balarabe, S. A., & Adeyemi, O. O. (2014). Hepatocurative potential of Vitex doniana root bark, stem bark and leaves extracts against CCl4-induced liver damage in rats. Asian Pacific journal of tropical biomedicine, 4(6), 480–485. https://doi.org/10.12980/APJTB.4.2014C207

Bunu, M. I., Ndinteh, D. T., Macdonald, J. R., Langat, M. K., Isyaka, S. M., Sadgrove, N. J., Melnikovova, I., & Fernandez-Cusimamani, E. (2021). Ecdysteroids from the Stem Bark of Vitex doniana Sweet (Lamiaceae; ex. Verbenaceae): A Geographically Variable African Medicinal Species. Antibiotics (Basel, Switzerland), 10(8), 937. https://doi.org/10.3390/antibiotics10080937

Carroll, J. J., Coburn, H., Douglass, R., & Babson, A. L. (1971). A simplified alkaline phosphotungstate assay for uric acid in serum. Clinical Chemistry, 17(3), 158–160. https://doi.org/10.1093/clinchem/17.3.158

Chen, T. K., Sperati, C. J., Thavarajah, S., & Grams, M. E. (2021). Reducing Kidney Function Decline in Patients With CKD: Core Curriculum 2021. American Journal of Kidney Diseases: The Official Journal of the National Kidney Foundation, 77(6), 969–983. https://doi.org/10.1053/j.ajkd.2020.12.022

Cohen, G., Dembiec, D., & Marcus, J. (1970). Measurement of catalase activity in tissue extracts. Analytical Biochemistry, 34(1), 30–38. https://doi.org/10.1016/0003-2697(70)90083-7

D’Armour, F. E., Blood, F. R., Belden, & D. A, (1965). The Manual for Laboratory Work in Mammalian Physiology. 3rd Ed. Illinois Chicago. The University of Chicago Press pp. 4-6.

Doumas, B. T., Watson, W. A., & Biggs, H. G. (1971). Albumin standards and the measurement of serum albumin with bromocresol green. Clinica Chimica Acta; International Journal of Clinical Chemistry, 31(1), 87–96. https://doi.org/10.1016/0009-8981(71)90365-2

Dubiwak, A. D., Damtew, T. W., Senbetu, M. W., Yewhalaw, D., Asere, T. G., Nemo, G., & Baye, M. F. (2021). Hepatoprotective effect of corm of Ensete ventricosum (Welw.) Cheesman extract against isoniazid and rifampicin induced hepatotoxicity in Swiss albino mice. Journal of Toxicology, 2021, 1–8. https://doi.org/10.1155/2021/4760455

Ellman, G. L. (1959). Tissue sulfhydryl groups. Archives of biochemistry and biophysics, 82(1), 70–77. https://doi.org/10.1016/0003-9861(59)90090-6

Fenech, G., & Tommasini, A. (1952). Metodo colorimetrico di dosaggio dell'urea [Method of colorimetric determination of urea]. Bollettino Chimico Farmaceutico, 91(10), 391– 395.

Gonnella, M., Rennaa, M., D’Imperio, M., Testonec, G., & Giannino, D. (2018). Phytochemicals in Asteraceae Leafy Vegetables. In: Spyridon, A., Petropoulos, I.C.F.R. and Ferreira, L.B., Eds., Phytochemicals in Vegetables: A Valuable Source of Bioactive Compounds. Bentham Science Sharjah, 166. https://doi.org/10.2174/9781681087399118010008

Hinson, J. A., Roberts, D. W., & James, L. P. (2010). Mechanisms of acetaminophen-induced liver necrosis. In Handbook of Experimental Pharmacology (pp. 369–405). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-00663-0_12

Hutson, D. H., Pickering, B. A., & Donninger, C. (1972). Phosphoric acid triester glutathione alkyltransferase. A mechanism for the detoxification of dimethyl phosphate triesters. The Biochemical Journal, 127(1), 285–293. https://doi.org/10.1042/bj1270285

Ikewuchi, J. C., Ikewuchi, C. C., & Ifeanacho, M. O. (2019). Nutrient and bioactive compounds composition of the leaves and stems of Pandiaka heudelotii: A wild vegetable. Heliyon, 5(4), e01501. https://doi.org/10.1016/j.heliyon.2019.e01501

Imoisi, C., Iyasele, J. U., & Okhale, S. E. (2021). Proximate and acute toxicity profile of Vitex doniana (black plum) fruit. Journal of Chemical Society of Nigeria, 46(2). https://doi.org/10.46602/jcsn.v46i2.597

Iyiola, A. O, & Adegoke Wahab, M. K. (2023). Herbal Medicine Methods and Practices in Nigeria. In: Izah, S.C., Ogwu, M.C., Akram, M. (eds) Herbal Medicine Phytochemistry. Reference Series in Phytochemistry. Springer, Cham. 2023. https://doi.org/10.1007/978-3-031-21973-3_47-1

Kamal, N., Mio Asni, N. S., Rozlan, I. N. A., Mohd Azmi, M. A. H., Mazlan, N. W., Mediani, A., Baharum, S. N., Latip, J., Assaw, S., & Edrada-Ebel, R. A. (2022). Traditional Medicinal Uses, Phytochemistry, Biological Properties, and Health Applications of Vitex sp. Plants (Basel, Switzerland), 11(15), 1944. https://doi.org/10.3390/plants11151944

Kennedy, G. L., Jr, Ferenz, R. L., & Burgess, B. A. (1986). Estimation of acute oral toxicity in rats by determination of the approximate lethal dose rather than the LD50. Journal of Applied Toxicology: JAT, 6(3), 145–148. https://doi.org/10.1002/jat.2550060302

Lorke D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54(4), 275–287. https://doi.org/10.1007/BF01234480

Lustgarten, J. A., & Wenk, R. E. (1972). Simple, rapid, kinetic method for serum creatinine measurement. Clinical Chemistry, 18(11), 1419–1422. https://doi.org/10.1093/clinchem/18.11.1419

Messaoudi, M., Rebiai, A., Sawicka, B., Atanassova, M., Ouakouak, H., Larkem, I., Egbuna, C., Awuchi, C. G., Boubekeur, S., Ferhat, M. A., Begaa, S., & Benchikha, N. (2021). Effect of Extraction Methods on Polyphenols, Flavonoids, Mineral Elements, and Biological Activities of Essential Oil and Extracts of Mentha pulegium L. Molecules (Basel, Switzerland), 27(1), 11. https://doi.org/10.3390/molecules27010011

Miller, L. C., & Tainter, M. (1994). Estimation of LD50 and its error by means of logarithmic-probit graph paper. Proced Socie Experiment Biology and Medicine, 57, 261–264. https://doi.org/10.3181/00379727-57-14776

Mohamed, A. A., Khalil, A. A., & El-Beltagi, H. E. S. (2010). Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grasas Y Aceites (1), 67–75. https://doi.dx.org/10.3989/gya.064509

Obasi, E., Iheanacho, K., Nwachukwu, N., Agha, N., & Chikezie, P. C. (2019). Evaluation of body weight, serum glucose level and oxidative stress parameters of diabetic rats administered phenolic aqueous leaf extract of Vitex doniana. Biomedical Research and Therapy, 6(9), 3359–3367. https://doi.org/10.15419/bmrat.v6i9.564

Onoja, S. O., Omeh, Y. N., Ezeja, M. I., & Chukwu, M. N. (2014). Evaluation of the in vitro and in vivo antioxidant potentials of Aframomum melegueta methanolic seed extract. Journal of Tropical Medicine, 2014, 159343. https://doi.org/10.1155/2014/159343

Onwukwe, O. S., Ukwuani, J. N., Onyemelukwe, A. O., Azubuike, N. C., Onuba, A. C., Ogu, O. C., Odo, O. F., & Achukwu, P. U. (2020). Assessment of the sub-acute toxicity profile of Vitex doniana (sweet) leaf fractions on albino rats. Annual Research & Review in Biology, 86–95. https://doi.org/10.9734/arrb/2020/v35i230192

Ouassou, H., Bouhrim, M., Daoudi, N. E., Mekhfi, H., Ziyyat, A., Legssyer, A., Aziz, M., & Bnouham, M. (2021). Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats. Advances in Pharmacological and Pharmaceutical Sciences, 2021, 8883040. https://doi.org/10.1155/2021/8883040

Pillai, S., Kobayashi, K., Michael, M., Mathai, T., Sivakumar, B., & Sadasivan, P. (2021). John William Trevan’s concept of Median Lethal Dose (LD50/LC50) – more misused than used. Journal of Pre-Clinical and Clinical Research, 15(3), 137–141. https://doi.org/10.26444/jpccr/139588

Rani, A., & Sharma, A. (2013). The genus Vitex: A review. Pharmacognosy Reviews, 7(14), 188–198. https://doi.org/10.4103/0973-7847.120522

The OECD guideline for testing of chemical: 420 Acute Oral Toxicity. France. (2010b). OECD Organization of Economic Cooperation and Development.

van der Sluis, A. A., Dekker, M., Verkerk, R., & Jongen, W. M. (2000). An improved, rapid in vitro method to measure antioxidant activity. Application On selected flavonoids and apple juice. Journal of Agricultural and Food Chemistry, 48(9), 4116–4122. https://doi.org/10.1021/jf000156i

Vunchi, M. A., Umar, A. N., King, M. A., Liman, A. A., Jeremiah, G., & Aigbe, C. O. (2011). Proximate, vitamins and mineral composition of Vitex doniana fruit pulp. Nigerian. Journal of Basic and Applied. Sciences, 19, 97–100. https://doi.org/10.4314/njbas.v19i1.69352

Watson, D., & Rogers, J. A. (1961). A study of six representative methods of plasma bilirubin analysis. Journal of Clinical Pathology, 14(3), 271–278. https://doi.org/10.1136/jcp.14.3.271

World Health Organization. (2000). General guidelines for methodologies on research and evaluation of traditional medicine. Switzerland.

Yin, K., Li, X., Luo, X., Sha, Y., Gong, P., Gu, J., & Tan, R. (2021). Hepatoprotective Effect and Potential Mechanism of Aqueous Extract from Phyllanthus emblica on Carbon-Tetrachloride-Induced Liver Fibrosis in Rats. Evidence-based Complementary and Alternative Medicine: eCAM, 2021, 5345821. https://doi.org/10.1155/2021/5345821

Yousef, M. I., Omar, S. A., El-Guendi, M. I., & Abdelmegid, L. A. (2010). Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food and Chemical Toxicology: An International Journal published for the British Industrial Biological Research Association, 48(11), 3246–3261. https://doi.org/10.1016/j.fct.2010.08.034

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Evaluation of hematological and biochemical parameters of liver function following consumption of Vitex doniana fruit syrup

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