The Inhibitory Effect of Selenium Supplementation on Tumor Progression in a DMBA-Induced Breast Cancer Model in Wistar Rats
Copyright © 2025 Nour EL-Houda Feriel Djebara , Adel Gouri, Houari Hemida, Chahinaise Zaoui, Bachir Benarba
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Background: Breast cancer remains a significant global public health concern, necessitating the ongoing exploration of novel preventive and therapeutic strategies. Selenium supplementation has been proposed as a potential chemopreventive agent, yet its efficacy lacks robust in vivo validation.
Aims: This study aimed to evaluate the chemopreventive potential of selenium supplementation and its effect on tumor progression in a 7,12-dimethylbenz[a]anthracene DMBA-induced breast cancer model in Wistar rats.
Methods: Twenty-four adult female Wister rat were allocated into four experimental groups (n=6): Control (vehicle only); DMBA (carcinogen control); DMBA + Se 200 µg/kg; and DMBA + Se 400 µg/kg. Mammary tumors were induced via a single intragastric administration of DMBA (80 mg/kg). Over a 23-week period, hematological, biochemical, and histopathological analyses were conducted. The volume of excised mammary tumors was measured post-sacrifice.
Results: Supplementation with selenium at a dose of 400 µg/kg resulted in a statistically significant reduction in mean tumor volume (0.13 cm³) compared to the DMBA-only group (1.32 cm³). Concurrently, this high-dose group exhibited significant amelioration in serum levels of specific biochemical markers including aspartate aminotransferase (AST), urea, and creatinine. Histopathological assessment further supported these findings, revealing a more preserved mammary tissue architecture in rats receiving the high-dose selenium.
Conclusions: While selenium supplementation at 400 µg/Kg demonstrated a significant inhibition effect on tumor progression and conferred hepatorenal protection, a definitive chemopreventive effect against DMBA-induced carcinogenesis was not established. These results indicate that selenium may function as a therapeutic modulator rather than a primary preventive agent in this model. Further investigation employing higher doses and alternative administration regimens is warranted to elucidate its full chemopreventive potential.
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Akhouri, V., Kumari, M., & Kumar, A. (2020). Therapeutic effect of Aegle marmelos fruit extract against DMBA induced breast cancer in rats. Scientific Reports, 10(1), 18016. https://doi.org/10.1038/s41598-020-72935-2
Baltaci, S. B., Mogulkoc, R., Baltaci, A. K., Emsen, A., & Artac, H. (2018). The effect of zinc and melatonin supplementation on immunity parameters in breast cancer induced by DMBA in rats. Archives of Physiology and Biochemistry, 124(3), 247–252. https://doi.org/10.1080/13813455.2017.1392580
Cai, X., Wang, C., Yu, W., Fan, W., Wang, S., Shen, N., Wu, P., Li, X., & Wang, F. (2016). Selenium exposure and cancer risk: An updated meta-analysis and meta-regression. Scientific Reports, 6(1), 19213. https://doi.org/10.1038/srep19213
Cardoso, B. R., Roberts, B. R., Bush, A. I., & Hare, D. J. (2015). Selenium, selenoproteins and neurodegenerative diseases. Metallomics. Integrated Biometal Science, 7(8), 1213–1228. https://doi.org/10.1039/c5mt00075k
Çay, M., & Naziroğlu, M. (1999). Effects of intraperitoneally-administered vitamin E and selenium on the blood biochemical and haematological parameters in rats. Cell Biochemistry and Function, 17(2), 143-148. https://doi.org/10.1002/(sici)1099-0844(199906)17:2<143::aid-cbf802>3.0.co;2-h
Charalabopoulos, K., Kotsalos, A., Batistatou, A., Charalabopoulos, A., Vezyraki, P., Peschos, D., Kalfakakou, V., & Evangelou, A. (2006). Selenium in serum and neoplastic tissue in breast cancer: correlation with CEA. British Journal of Cancer, 95(6), 674–676. https://doi.org/10.1038/sj.bjc.6603292
Cinato, M., Andersson, L., Miljanovic, A., Laudette, M., Kunduzova, O., Borén, J., & Levin, M. C. (2024). Role of perilipins in oxidative stress-implications for cardiovascular disease. Antioxidants (Basel, Switzerland), 13(2), 209. https://doi.org/10.3390/antiox13020209
El-Bayoumy, K. (2001). The protective role of selenium on genetic damage and on cancer. Mutation Research, 475(1–2), 123–139. https://doi.org/10.1016/s0027-5107(01)00075-6
El makawy, A. I., Mabrouk, D. M., Mohammed, S. E., Abdel-Aziem, S. H., EL-Kader, H. A. A., Sharaf, H. A., Youssef, D. A., & Ibrahim, F. M. (2022). The suppressive role of nanoencapsulated chia oil against DMBA-induced breast cancer through oxidative stress repression and tumor genes expression modulation in rats. Molecular Biology Reports, 49(11), 10217–10228. https://doi.org/10.1007/s11033-022-07885-1
Elen, A., & Turan, M. K. (2019). Classifying white blood cells using machine learning algorithms. Uluslararası Muhendislik Arastirma ve Gelistirme Dergisi, 141–152. https://doi.org/10.29137/umagd.498372
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